Base composition of intact nucleic acid oligomers
نویسندگان
چکیده
منابع مشابه
Purification of intact nucleic acid from samples
is required for many molecular biology applications. Accurate PCR replication of RNA and DNA sequences requires complete removal of cellular lipids and proteins. Likewise, most restriction endonucleases used to digest genomic DNA are inactivated or degraded by cellular proteases normally present prior to purification of the nucleic acids. Thus, failure to remove these cellular contaminants ofte...
متن کاملTargeting linear duplex DNA with mixed-base peptide nucleic acid oligomers facilitated by bisPNA openers.
PD-loop complexes formed by bisPNA openers and a mixed-base (mb) oligonucleotide on double-stranded (ds) DNA enable various diagnostic and biotechnological manipulations with linear DNA duplexes [1–5]. So far, the PD-loop formation requires the use of two homopyrimidine bisPNA oligomers to open the DNA duplex for binding an oligonucleotide [6]. This condition imposes certain sequence limitation...
متن کاملFluorescent nucleic acid base analogues.
The use of fluorescent nucleic acid base analogues is becoming increasingly important in the fields of biology, biochemistry and biophysical chemistry as well as in the field of DNA nanotechnology. The advantage of being able to incorporate a fluorescent probe molecule close to the site of examination in the nucleic acid-containing system of interest with merely a minimal perturbation to the na...
متن کاملLocked nucleic acid oligomers as handles for single molecule manipulation
Single-molecule manipulation (SMM) techniques use applied force, and measured elastic response, to reveal microscopic physical parameters of individual biomolecules and details of biomolecular interactions. A major hurdle in the application of these techniques is the labeling method needed to immobilize biomolecules on solid supports. A simple, minimally-perturbative labeling strategy would sig...
متن کاملDetermination of the backbone structure of nucleic acids and nucleic acid oligomers by laser Raman scattering.
Raman spectra of fibers of DNA that have been prepared in the A, B, and C forms are presented and compared with Raman spectra of DNA and RNA in dilute solution. It is shown that the phosphate vibrations in the region 750-850 cm(-1) are very sensitive to the specific conformation of the phosphate group in the backbone chain and are virtually independent of all ohter factors. Thus, a very simple ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biopolymers
سال: 1966
ISSN: 0006-3525,1097-0282
DOI: 10.1002/bip.1966.360040706